SunCatcher thus provides a rapid and sensitive approach for studying live single-cell clones and clonal evolution, and performing functional analyses. Early spontaneous metastases can also be identified and quantified. While the heterogeneous BC pools reproduce their original parental cell line proliferation and tumor progression rates, individual BCs are phenotypically and functionally diverse. We apply SunCatcher to various breast cancer cell lines and combine respective BCs to create versions of the original cell lines. We develop quantitative PCR-based and next-generation sequencing methods that we employ to identify and quantify BCs in vitro and in vivo. From complex cell populations, we generate single cell-derived clonal populations, infect each with a unique molecular barcode, and retain stocks of individual barcoded clones (BCs). We report a clonal molecular barcoding method, called SunCatcher, that enables longitudinal tracking and live cell functional analysis. That is a particular issue when further study of cells that were eliminated during experimentation could provide critical information. Single-cell analysis methods are valuable tools however, current approaches do not easily enable live cell retrieval.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |